MICROSCOPY TECHNIQUES AND PROKARYOTE

This week (week 3), in microbiology class I identified the parts of a basic microscope. Besides, I described the principle of light, phase-contrast, fluorescence and electron microscope. Furthermore, I have to select suitable microscope for relevant usage and recognized images produces from different microscope.

MICROSCOPE PARTS 

TYPES OF MICROSCOPE:

OPTICAL

- dark field

- bright field

- fluorescence microscopy

- phase-contrast microscopy

ELECTRON

- scanning electron microscope

- transmission electron microscope

- environmental scanning electron microscope

SCANNING PROBE

- atomic force

- scanning tunneling

- scanning near-field optical microscope

SCANNING ACOUSTIC MICROSCOPE

PROKARYOTE

For Thursday Class, Dr Wan asked every student to make online mind map and submit before class. This mind map was very interesting and useful as can put video, picture and article, so student can study based on this mind map. It easy to understand and remember. Besides. during class, Dr Wan asked everybody to draw a mind map on the white board. This part was very funny as everyone busy to write everything about prokaryote so there have same answer. At end of class, Dr wan asked students to make a quizlet about prokaryote. There we did some word and explanation than shared it with everybody. We also did a test based on what we had done in the quizlet.

In this week or this topics, I very enjoy and happy with the class as I'm not sleepy in the class but I very concentrate with the learning. This Microbiology class was awesome.

INTRODUCTION OF MICROBIOLOGY

WHAT IS MICROBIOLOGY? 

The study of microscopic organism such as, bacteria, virus, fungi , protozoa and archaea which cannot be seen by naked eyes but can be seen by using microscope.

Thiomargarita namibiensis, a giant bacteria in the world found in
 the ocean sediments of the continental shelf  of  Namibia, Africa

Application in Microbiology
-production of antibiotics 
-use in industrial such as bread,cheese etc
-provide numerous drugs
-act as natural insect killer in garden
-nitrogen fixation
-used as thickening and stabilizing agent such as in water based paint and cosmetic

Bacillus thuringiensis, used as biological pesticide for insecticides

Penicillium produce penicillin used as antibiotic also used for
cheesemaking

Watermelon snow or snow algae is Chlamydomonas nivalis 



Deinococcus radiodurans is an extremophilic bacterium called as
canon of bacteria which resistance of radiation, vacuum, acid and also
survive in cold.

Bacillus anthracis is agent of anthrax 

Bacteria as Art
Biophysicists are growing Petri dishes of different species of bacteria in order to develop new antibiotic. The bacteria are subjected to different temperatures and have limited sources of food inside the dish. This cause most colonies to communicate and reproduce. Their growth results in unique patterns of varying colors a sort of "bacteria painting".  







How "Bacteria Talk"
Bacteria grow twice their size by cut themselves down in the middle and become two and continue. They also a single cell microscopic organisms. Besides, they only have a piece of DNA, very few of genes and genetic information to encodes all the traits that they carry out. All bacteria can talk to each other by making chemical words and recognize those words then turn on group behaviors called a quorum sensing.





  

Pleomorphic

- the bacteria have ability to alter shape or size in response to environment condition example Deinococcaceae family

- have lack of cell wall

Microplasma

- the smallest organism in the world

- can be generated at a variety of temperature and pressure, existing as either thermal or non-thermal

- wall-less which is lack of cell wall

 Thermoplasma

- genus of archaea which is thrive in acidic and high-temperature environments

- respire using sulfur and organic carbon 

- do not contain cell wall

Vampyrellid

- species attacks Mougeotia cells

- makes a large hole in the algal cell wall